Molecular Characterization of Toxigenic Clostridium difficile in a Northern Italian Hospital
نویسندگان
چکیده
منابع مشابه
Molecular Analysis of Toxigenic Clostridium difficile Isolates from Hospital Environment by PCR Ribotyping Method
Background and Aims: Clostridium difficile is an identified cause of antibiotic-associated diarrhea, antibiotic-associated colitis, pseudomembranous colitis and nosocomial diarrhea. The objective of this survey was to determine molecular analysis of toxigenic Clostridium difficile isolates from hospital environment in Tehran tertiary medical centers. Materials and Methods: In this descriptiv...
متن کاملComparison of two molecular methods for detecting toxigenic Clostridium difficile.
BACKGROUND Clostridium difficile is one of the most common causes of nosocomial diarrhea, and diagnostic methods for detecting C. difficile infection have shifted from conventional to more recent molecular techniques. This study aimed to compare the performance of two molecular assays (Meridian Illumigene™ and AdvanSure CD real-time PCR) in detecting C. difficile using a toxigenic culture as a ...
متن کاملIdentification of toxigenic Clostridium difficile by counterimmunoelectrophoresis.
A counterimmunoelectrophoresis (CIE) technique which reacted positively with culture filtrates of Clostridium difficile was developed and compared with a cytotoxicity assay in human embryonic lung cell cultures. CIE, employing C. sordellii antitoxin, detected 17 of 17 C. difficile strains. Of those positive by CIE, 13 were cytotoxic in cell culture. Fourteen Clostridium species other than C. di...
متن کاملCommunity Environmental Contamination of Toxigenic Clostridium difficile
BACKGROUND Clostridium difficile infection is often considered to result from recent acquisition of a C difficile isolate in a healthcare setting. However, C difficile spores can persist for long periods of time, suggesting a potentially large community environmental reservoir. The objectives of this study were to assess community environmental contamination of toxigenic C difficile and to asse...
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ژورنال
عنوان ژورنال: Current Microbiology
سال: 2014
ISSN: 0343-8651,1432-0991
DOI: 10.1007/s00284-014-0690-9